Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 170272..170915 Replicon plasmid pLSH-KPN148-1
Accession NZ_CP040123
Organism Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FDY04_RS27380 Protein ID WP_001044770.1
Coordinates 170499..170915 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FDY04_RS27375 Protein ID WP_001261282.1
Coordinates 170272..170502 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDY04_RS27345 165495..166274 - 780 WP_013214009.1 site-specific integrase -
FDY04_RS27350 166458..167462 - 1005 WP_011977814.1 hypothetical protein -
FDY04_RS27355 167492..167695 - 204 WP_011977813.1 hypothetical protein -
FDY04_RS27360 167741..168262 - 522 WP_013214008.1 hypothetical protein -
FDY04_RS27365 168320..168712 - 393 WP_011977811.1 hypothetical protein -
FDY04_RS27370 168749..169693 - 945 WP_011977810.1 hypothetical protein -
FDY04_RS28830 169854..170315 - 462 WP_014343465.1 hypothetical protein -
FDY04_RS27375 170272..170502 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FDY04_RS27380 170499..170915 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FDY04_RS27385 170989..172551 + 1563 WP_004206609.1 AAA family ATPase -
FDY04_RS27390 172536..173558 + 1023 WP_000361404.1 helicase UvrD -
FDY04_RS27395 173814..174511 + 698 Protein_202 IS1 family transposase -
FDY04_RS27400 174540..174812 + 273 Protein_203 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaSHV-12 / sul2 - 1..227415 227415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T125013 WP_001044770.1 NZ_CP040123:170499-170915 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T125013 NZ_CP040123:170499-170915 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT125013 WP_001261282.1 NZ_CP040123:170272-170502 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT125013 NZ_CP040123:170272-170502 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References