Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1203..1939 Replicon plasmid pLSH-KPN148-1
Accession NZ_CP040123
Organism Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FDY04_RS26415 Protein ID WP_003026803.1
Coordinates 1203..1685 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FDY04_RS26420 Protein ID WP_003026799.1
Coordinates 1673..1939 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDY04_RS26410 76..1002 - 927 Protein_0 ISNCY-like element ISKpn21 family transposase -
FDY04_RS26415 1203..1685 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FDY04_RS26420 1673..1939 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FDY04_RS26425 2115..2369 - 255 WP_004152108.1 hypothetical protein -
FDY04_RS26430 2445..2702 - 258 WP_004152107.1 hypothetical protein -
FDY04_RS26435 2751..2954 - 204 WP_004152106.1 hemolysin expression modulator Hha -
FDY04_RS26440 2988..3356 - 369 WP_004152105.1 hypothetical protein -
FDY04_RS26445 3400..3894 - 495 WP_004152104.1 DNA-binding protein -
FDY04_RS26450 3925..4500 - 576 WP_004152103.1 hypothetical protein -
FDY04_RS26455 4488..4757 - 270 WP_004152102.1 hypothetical protein -
FDY04_RS26460 5115..5465 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
FDY04_RS26465 5515..5877 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaSHV-12 / sul2 - 1..227415 227415
- flank IS/Tn - - 46..1002 956


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T125012 WP_003026803.1 NZ_CP040123:c1685-1203 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T125012 NZ_CP040123:c1685-1203 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT125012 WP_003026799.1 NZ_CP040123:c1939-1673 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT125012 NZ_CP040123:c1939-1673 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References