Detailed information of TA system TA125012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1203..1939
• Replicon: plasmid pLSH-KPN148-1
• Accession: NZ_CP040123
• Organism: Klebsiella pneumoniae strain LSH-KPN148

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: FDY04_RS26415
• Protein ID: WP_003026803.1
• Coordinates: 1203..1685 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FDY04_RS26420
• Protein ID: WP_003026799.1
• Coordinates: 1673..1939 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDY04_RS26410	76..1002	-	927	Protein_0	ISNCY-like element ISKpn21 family transposase	-
FDY04_RS26415	1203..1685	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
FDY04_RS26420	1673..1939	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FDY04_RS26425	2115..2369	-	255	WP_004152108.1	hypothetical protein	-
FDY04_RS26430	2445..2702	-	258	WP_004152107.1	hypothetical protein	-
FDY04_RS26435	2751..2954	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
FDY04_RS26440	2988..3356	-	369	WP_004152105.1	hypothetical protein	-
FDY04_RS26445	3400..3894	-	495	WP_004152104.1	DNA-binding protein	-
FDY04_RS26450	3925..4500	-	576	WP_004152103.1	hypothetical protein	-
FDY04_RS26455	4488..4757	-	270	WP_004152102.1	hypothetical protein	-
FDY04_RS26460	5115..5465	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FDY04_RS26465	5515..5877	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaSHV-12 / sul2	-	1..227415	227415
	-	flank	IS/Tn	-	-	46..1002	956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T125012 WP_003026803.1 NZ_CP040123:c1685-1203 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T125012 NZ_CP040123:c1685-1203 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT125012 WP_003026799.1 NZ_CP040123:c1939-1673 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT125012 NZ_CP040123:c1939-1673 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66