Detailed information of TA system TA124993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 386..878
• Replicon: plasmid pMB9_3
• Accession: NZ_CP040110
• Organism: Escherichia coli O157:H7 strain MB9-1

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: FDZ86_RS27945
• Protein ID: WP_000702246.1
• Coordinates: 618..878 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: FDZ86_RS27940
• Protein ID: WP_021558914.1
• Coordinates: 386..628 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ86_RS27940	386..628	-	243	WP_021558914.1	transcriptional regulator	Antitoxin
FDZ86_RS27945	618..878	-	261	WP_000702246.1	hypothetical protein	Toxin
FDZ86_RS27950	930..1154	-	225	WP_000814384.1	hypothetical protein	-
FDZ86_RS27955	1251..1460	-	210	WP_000866037.1	hypothetical protein	-
FDZ86_RS27960	1501..1824	-	324	WP_000432876.1	hypothetical protein	-
FDZ86_RS27965	3530..4543	+	1014	WP_024176825.1	replication initiation protein	-
FDZ86_RS27970	4666..4935	+	270	WP_000190069.1	hypothetical protein	-
FDZ86_RS27975	5240..5470	+	231	WP_001043556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37889	37889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T124993 WP_000702246.1 NZ_CP040110:c878-618 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T124993 NZ_CP040110:c878-618 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9258.66 Da
• Isoelectric Point: 9.6823

>AT124993 WP_021558914.1 NZ_CP040110:c628-386 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT124993 NZ_CP040110:c628-386 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7