Detailed information of TA system TA124988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 74801..75360
• Replicon: plasmid pMB9_1
• Accession: NZ_CP040108
• Organism: Escherichia coli O157:H7 strain MB9-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: FDZ86_RS27810
• Protein ID: WP_000421248.1
• Coordinates: 75085..75360 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: FDZ86_RS27805
• Protein ID: WP_001178089.1
• Coordinates: 74801..75085 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ86_RS27765	69993..70553	+	561	WP_000840472.1	fertility inhibition protein FinO	-
FDZ86_RS27770	70686..70898	+	213	WP_001302189.1	ANR family transcriptional regulator	-
FDZ86_RS27775	71143..71604	+	462	WP_001233853.1	thermonuclease family protein	-
FDZ86_RS27780	71650..71859	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
FDZ86_RS27785	71897..72235	+	339	WP_000766796.1	hypothetical protein	-
FDZ86_RS27790	72475..72729	+	255	WP_000083831.1	replication regulatory protein RepA	-
FDZ86_RS27795	72965..73039	+	75	WP_001370046.1	RepA leader peptide Tap	-
FDZ86_RS27800	73032..73889	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
FDZ86_RS27805	74801..75085	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
FDZ86_RS27810	75085..75360	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDZ86_RS27815	75455..75661	+	207	WP_001105064.1	hypothetical protein	-
FDZ86_RS27820	75761..75976	-	216	Protein_82	transposase	-
FDZ86_RS27825	76020..76586	-	567	Protein_83	IS3-like element IS600 family transposase	-
FDZ86_RS27830	76586..77002	-	417	Protein_84	IS91 family transposase	-
FDZ86_RS27835	77002..77187	-	186	WP_001302179.1	hypothetical protein	-
FDZ86_RS27840	77364..79574	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
FDZ86_RS27845	79618..80007	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP	1..93190	93190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T124988 WP_000421248.1 NZ_CP040108:75085-75360 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T124988 NZ_CP040108:75085-75360 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT124988 WP_001178089.1 NZ_CP040108:74801-75085 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT124988 NZ_CP040108:74801-75085 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8