Detailed information of TA system TA124970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2578328..2578799
• Replicon: chromosome
• Accession: NZ_CP040107
• Organism: Escherichia coli O157:H7 strain MB9-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FDZ86_RS13000
• Protein ID: WP_001303511.1
• Coordinates: 2578328..2578606 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FDZ86_RS13005
• Protein ID: WP_001302048.1
• Coordinates: 2578608..2578799 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ86_RS12960	2573334..2573518	-	185	Protein_2530	DUF3596 domain-containing protein	-
FDZ86_RS12965	2573553..2573804	-	252	WP_000005552.1	excisionase family protein	-
FDZ86_RS12970	2573877..2576348	-	2472	WP_000048458.1	exonuclease	-
FDZ86_RS12975	2576441..2576632	-	192	WP_001090200.1	DUF1482 family protein	-
FDZ86_RS12980	2576629..2576817	-	189	WP_000449192.1	cell division inhibition protein DicB	-
FDZ86_RS12985	2577218..2577382	+	165	WP_001331716.1	hypothetical protein	-
FDZ86_RS12990	2577386..2577604	-	219	WP_001171930.1	hypothetical protein	-
FDZ86_RS12995	2577676..2577975	-	300	WP_001240334.1	hypothetical protein	-
FDZ86_RS13000	2578328..2578606	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FDZ86_RS13005	2578608..2578799	-	192	WP_001302048.1	hypothetical protein	Antitoxin
FDZ86_RS13010	2578820..2579191	-	372	WP_001169686.1	hypothetical protein	-
FDZ86_RS13015	2579289..2579591	+	303	WP_000172738.1	transcriptional regulator	-
FDZ86_RS13020	2579588..2580013	+	426	WP_000693943.1	Rha family transcriptional regulator	-
FDZ86_RS13025	2580036..2580998	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
FDZ86_RS13030	2581005..2581745	+	741	WP_000788936.1	ATP-binding protein	-
FDZ86_RS13035	2581771..2582540	+	770	Protein_2545	DUF1627 domain-containing protein	-
FDZ86_RS13040	2582556..2582951	+	396	WP_001118159.1	DUF977 family protein	-
FDZ86_RS13045	2583008..2583592	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2571167..2626173	55006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T124970 WP_001303511.1 NZ_CP040107:c2578606-2578328 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T124970 NZ_CP040107:c2578606-2578328 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT124970 WP_001302048.1 NZ_CP040107:c2578799-2578608 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT124970 NZ_CP040107:c2578799-2578608 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0