Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57708..58233 Replicon plasmid pFT130-2
Accession NZ_CP040092
Organism Escherichia coli strain FT130

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FC325_RS23295 Protein ID WP_001159871.1
Coordinates 57708..58013 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FC325_RS23300 Protein ID WP_000813634.1
Coordinates 58015..58233 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC325_RS23260 53686..54357 - 672 WP_046788564.1 FadR family transcriptional regulator -
FC325_RS23265 54452..54994 - 543 Protein_70 GGDEF domain-containing protein -
FC325_RS23270 55011..55697 - 687 Protein_71 IS1 family transposase -
FC325_RS23275 55754..56323 + 570 Protein_72 lipid II-degrading bacteriocin colicin M -
FC325_RS23280 56373..56726 - 354 WP_000864812.1 colicin M immunity protein -
FC325_RS23285 56673..56797 - 125 Protein_74 colicin immunity protein -
FC325_RS23290 56898..57707 - 810 WP_000016963.1 site-specific integrase -
FC325_RS23295 57708..58013 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FC325_RS23300 58015..58233 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FC325_RS23305 58701..59396 + 696 WP_072652234.1 DNA-binding protein -
FC325_RS23310 59393..60013 + 621 WP_072652229.1 hypothetical protein -
FC325_RS23315 60010..60798 + 789 WP_072652230.1 DUF2971 domain-containing protein -
FC325_RS23320 60834..61574 + 741 WP_000933099.1 abortive infection family protein -
FC325_RS23325 61733..62362 - 630 Protein_82 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / qnrS1 / floR - 1..63136 63136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T124924 WP_001159871.1 NZ_CP040092:c58013-57708 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T124924 NZ_CP040092:c58013-57708 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT124924 WP_000813634.1 NZ_CP040092:c58233-58015 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT124924 NZ_CP040092:c58233-58015 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References