Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3450193..3450811 Replicon chromosome
Accession NZ_CP040090
Organism Escherichia coli strain FT130

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FC325_RS16635 Protein ID WP_001291435.1
Coordinates 3450593..3450811 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FC325_RS16630 Protein ID WP_000344800.1
Coordinates 3450193..3450567 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC325_RS16620 3445282..3446475 + 1194 WP_089046497.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FC325_RS16625 3446498..3449647 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FC325_RS16630 3450193..3450567 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FC325_RS16635 3450593..3450811 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FC325_RS16640 3450983..3451534 + 552 WP_000102564.1 maltose O-acetyltransferase -
FC325_RS16645 3451650..3452120 + 471 WP_000136192.1 YlaC family protein -
FC325_RS16650 3452284..3453834 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FC325_RS16655 3453876..3454229 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FC325_RS16665 3454608..3454919 + 312 WP_000409911.1 MGMT family protein -
FC325_RS16670 3454950..3455522 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T124918 WP_001291435.1 NZ_CP040090:3450593-3450811 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T124918 NZ_CP040090:3450593-3450811 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT124918 WP_000344800.1 NZ_CP040090:3450193-3450567 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT124918 NZ_CP040090:3450193-3450567 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References