Detailed information of TA system TA124900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2309..2897
• Replicon: plasmid unnamed1
• Accession: NZ_CP040088
• Organism: Acinetobacter baumannii strain VB35575

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0J8TIH5
• Locus tag: FDN01_RS19545
• Protein ID: WP_000438827.1
• Coordinates: 2309..2596 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: FDN01_RS19550
• Protein ID: WP_001983304.1
• Coordinates: 2583..2897 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDN01_RS19530	1..936	+	936	WP_001292329.1	replication initiation protein RepM	-
FDN01_RS19535	1014..1532	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
FDN01_RS19540	1742..2041	+	300	WP_000358348.1	hypothetical protein	-
FDN01_RS19545	2309..2596	+	288	WP_000438827.1	BrnT family toxin	Toxin
FDN01_RS19550	2583..2897	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
FDN01_RS19555	3025..5436	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
FDN01_RS19560	5741..6203	+	463	Protein_6	DIP1984 family protein	-
FDN01_RS19565	6529..6738	-	210	WP_000069474.1	hypothetical protein	-
FDN01_RS19570	6731..7033	-	303	WP_001129974.1	XRE family transcriptional regulator	-
FDN01_RS19575	7026..7382	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11194	11194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T124900 WP_000438827.1 NZ_CP040088:2309-2596 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T124900 NZ_CP040088:2309-2596 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT124900 WP_001983304.1 NZ_CP040088:2583-2897 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT124900 NZ_CP040088:2583-2897 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0J8TIH5

Antitoxin

• AlphaFold DB: N9M5I3