Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49776..50301 Replicon plasmid p_unnamed3
Accession NZ_CP040070
Organism Escherichia coli strain A1_181

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FDP43_RS25590 Protein ID WP_001159868.1
Coordinates 49776..50081 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FDP43_RS25595 Protein ID WP_000813634.1
Coordinates 50083..50301 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDP43_RS25570 46127..46396 - 270 WP_001241949.1 RepB family plasmid replication initiator protein -
FDP43_RS25575 46453..47666 + 1214 WP_180707482.1 IS3 family transposase -
FDP43_RS25580 47707..48195 - 489 Protein_60 RepB family plasmid replication initiator protein -
FDP43_RS25585 48969..49775 - 807 WP_000016982.1 site-specific integrase -
FDP43_RS25590 49776..50081 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FDP43_RS25595 50083..50301 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FDP43_RS25600 50935..51132 + 198 WP_000215657.1 hypothetical protein -
FDP43_RS25605 51129..51413 - 285 WP_000642771.1 hypothetical protein -
FDP43_RS25610 51433..52529 - 1097 Protein_66 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA5 - 1..82718 82718
- inside IScluster/Tn - - 46453..56279 9826


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T124880 WP_001159868.1 NZ_CP040070:c50081-49776 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T124880 NZ_CP040070:c50081-49776 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT124880 WP_000813634.1 NZ_CP040070:c50301-50083 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT124880 NZ_CP040070:c50301-50083 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References