Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5304734..5305359 Replicon chromosome
Accession NZ_CP040028
Organism Klebsiella pneumoniae strain KPC160121

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FDK25_RS26735 Protein ID WP_002882817.1
Coordinates 5304734..5305117 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FDK25_RS26740 Protein ID WP_004150355.1
Coordinates 5305117..5305359 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDK25_RS26720 5302100..5303002 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FDK25_RS26725 5302999..5303634 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FDK25_RS26730 5303631..5304560 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FDK25_RS26735 5304734..5305117 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FDK25_RS26740 5305117..5305359 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FDK25_RS26745 5305564..5306481 + 918 WP_108714542.1 alpha/beta hydrolase -
FDK25_RS26750 5306495..5307436 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FDK25_RS26755 5307481..5307918 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FDK25_RS26760 5307915..5308775 - 861 WP_002882807.1 virulence factor BrkB family protein -
FDK25_RS26765 5308769..5309368 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124798 WP_002882817.1 NZ_CP040028:c5305117-5304734 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124798 NZ_CP040028:c5305117-5304734 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124798 WP_004150355.1 NZ_CP040028:c5305359-5305117 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124798 NZ_CP040028:c5305359-5305117 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References