Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53668..54404 Replicon plasmid pKpn3-L132
Accession NZ_CP040025
Organism Klebsiella pneumoniae strain KPC160132

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FDK24_RS28545 Protein ID WP_003026803.1
Coordinates 53922..54404 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FDK24_RS28540 Protein ID WP_003026799.1
Coordinates 53668..53934 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FDK24_RS28495 49730..50092 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
FDK24_RS28500 50142..50492 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
FDK24_RS28505 50850..51119 + 270 WP_004152102.1 hypothetical protein -
FDK24_RS28510 51107..51682 + 576 WP_004152103.1 hypothetical protein -
FDK24_RS28515 51713..52207 + 495 WP_004152104.1 DNA-binding protein -
FDK24_RS28520 52251..52619 + 369 WP_004152105.1 hypothetical protein -
FDK24_RS28525 52653..52856 + 204 WP_004152106.1 hemolysin expression modulator Hha -
FDK24_RS28530 52905..53162 + 258 WP_004152107.1 hypothetical protein -
FDK24_RS28535 53238..53492 + 255 WP_004152108.1 hypothetical protein -
FDK24_RS28540 53668..53934 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FDK24_RS28545 53922..54404 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FDK24_RS28550 54605..56008 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
FDK24_RS28555 56037..56669 - 633 WP_001567369.1 hypothetical protein -
FDK24_RS28560 57104..58108 - 1005 Protein_57 IS110 family transposase -
FDK24_RS28565 58187..58744 - 558 WP_003100847.1 recombinase family protein -
FDK24_RS28570 58738..59109 - 372 WP_003100853.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaDHA-1 / qnrB4 / catA1 - 1..150325 150325


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T124780 WP_003026803.1 NZ_CP040025:53922-54404 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T124780 NZ_CP040025:53922-54404 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT124780 WP_003026799.1 NZ_CP040025:53668-53934 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT124780 NZ_CP040025:53668-53934 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References