Detailed information of TA system TA124744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2166859..2167424
• Replicon: chromosome
• Accession: NZ_CP040007
• Organism: Arachnia propionica strain F0700

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A4P8ZSL9
• Locus tag: FBF34_RS09595
• Protein ID: WP_014846982.1
• Coordinates: 2166859..2167206 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FBF34_RS09600
• Protein ID: WP_041697619.1
• Coordinates: 2167206..2167424 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF34_RS09590	2163763..2165793	+	2031	WP_123824201.1	hypothetical protein	-
FBF34_RS09595	2166859..2167206	-	348	WP_014846982.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FBF34_RS09600	2167206..2167424	-	219	WP_041697619.1	ribbon-helix-helix domain-containing protein	Antitoxin
FBF34_RS09605	2167571..2170420	-	2850	WP_014846984.1	vitamin B12-dependent ribonucleotide reductase	-
FBF34_RS09610	2170458..2170994	-	537	WP_014846985.1	transcriptional regulator NrdR	-
FBF34_RS09615	2171193..2171522	-	330	WP_123824199.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-37)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12461.49 Da
• Isoelectric Point: 11.7369

>T124744 WP_014846982.1 NZ_CP040007:c2167206-2166859 [Arachnia propionica]
VLRGEIRLVDLEPARAGEADKRRPAIIVSNNRANTVAARLGRGVVTVVPVTSNTARVFPFQVLLTADEVGIRLDSKAQAE
QVRAVSVDRIGPVIGRLPARLVAQLDEALRLHLQL

Nucleotide

• Length: 348 bp

>T124744 NZ_CP040007:c2167206-2166859 [Arachnia propionica]
GTGCTCCGCGGCGAGATCCGGCTGGTCGATCTGGAGCCCGCACGGGCGGGAGAGGCAGACAAGCGTCGTCCGGCGATCAT
CGTCAGCAACAACCGCGCCAACACCGTCGCCGCTCGACTCGGGCGAGGCGTGGTCACGGTCGTCCCCGTCACCAGTAATA
CCGCGCGGGTCTTTCCGTTTCAGGTGCTTCTCACCGCGGATGAGGTCGGCATCCGCTTGGATTCCAAGGCCCAGGCCGAA
CAGGTCCGAGCGGTGTCAGTCGACCGCATCGGCCCGGTGATCGGGCGCCTTCCCGCCCGCCTGGTCGCCCAACTGGATGA
GGCGCTCCGGCTGCACCTTCAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7591.42 Da
• Isoelectric Point: 4.0390

>AT124744 WP_041697619.1 NZ_CP040007:c2167424-2167206 [Arachnia propionica]
VKLSVSLSPSEVAVLDKYAQSAGLKSRSAAIQQAIKLLGDPDLEDAYTAAWQEWEDAGEAEAWAATVADGVK

Nucleotide

• Length: 219 bp

>AT124744 NZ_CP040007:c2167424-2167206 [Arachnia propionica]
GTGAAGTTGAGCGTGAGTCTGTCGCCGAGCGAGGTCGCCGTGCTCGACAAGTACGCACAGTCTGCGGGTTTGAAGTCACG
ATCGGCGGCGATCCAACAGGCCATCAAGCTGCTGGGCGATCCCGACCTGGAGGATGCCTACACCGCTGCCTGGCAGGAGT
GGGAGGACGCCGGAGAGGCCGAGGCCTGGGCGGCCACCGTCGCCGACGGGGTGAAGTAG

Structures

Toxin

• AlphaFold DB: A0A4P8ZSL9