Detailed information of TA system TA124727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2045206..2045735
• Replicon: chromosome
• Accession: NZ_CP039992
• Organism: Staphylococcus aureus strain Lr3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E1E63_RS10255
• Protein ID: WP_000621175.1
• Coordinates: 2045206..2045568 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E1E63_RS10260
• Protein ID: WP_000948331.1
• Coordinates: 2045565..2045735 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1E63_RS10235	2042185..2042955	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
E1E63_RS10240	2042930..2043409	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E1E63_RS10245	2043411..2043737	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E1E63_RS10250	2043856..2044857	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E1E63_RS10255	2045206..2045568	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E1E63_RS10260	2045565..2045735	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E1E63_RS10265	2045820..2046968	-	1149	WP_001281154.1	alanine racemase	-
E1E63_RS10270	2047034..2047393	-	360	WP_000581197.1	holo-ACP synthase	-
E1E63_RS10275	2047397..2047888	-	492	WP_001205912.1	PH domain-containing protein	-
E1E63_RS10280	2047875..2049458	-	1584	WP_001294620.1	PH domain-containing protein	-
E1E63_RS10285	2049451..2049930	-	480	WP_001287079.1	hypothetical protein	-
E1E63_RS10290	2050139..2050699	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T124727 WP_000621175.1 NZ_CP039992:c2045568-2045206 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T124727 NZ_CP039992:c2045568-2045206 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT124727 WP_000948331.1 NZ_CP039992:c2045735-2045565 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT124727 NZ_CP039992:c2045735-2045565 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1