Detailed information of TA system TA124721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): slvAT/RES(toxin)
• Location: 147817..148733
• Replicon: plasmid pBT2101
• Accession: NZ_CP039991
• Organism: Pseudomonas aeruginosa strain T2101

Toxin (Protein)

• Gene name: slvT
• Uniprot ID: A0A2L1KI32
• Locus tag: FDK04_RS31710
• Protein ID: WP_019437783.1
• Coordinates: 147817..148341 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: slvA
• Uniprot ID: A0A2L1KH13
• Locus tag: FDK04_RS31715
• Protein ID: WP_019437782.1
• Coordinates: 148338..148733 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDK04_RS31690	143083..144228	+	1146	WP_021263240.1	glycerate kinase	-
FDK04_RS31695	144703..144894	-	192	WP_019437785.1	hypothetical protein	-
FDK04_RS31700	145269..146324	+	1056	WP_109392701.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
FDK04_RS31705	146521..147771	+	1251	WP_019437784.1	TerD family protein	-
FDK04_RS31710	147817..148341	-	525	WP_019437783.1	RES family NAD+ phosphorylase	Toxin
FDK04_RS31715	148338..148733	-	396	WP_019437782.1	hypothetical protein	Antitoxin
FDK04_RS31720	149317..151236	-	1920	WP_021263737.1	hypothetical protein	-
FDK04_RS31725	151400..151654	+	255	WP_021263738.1	hypothetical protein	-
FDK04_RS31730	152548..153072	+	525	WP_010794447.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCARB-2 / qacE / floR / tet(G) / sul1 / blaOXA-10 / ant(3'')-Ia / blaVEB-1 / ant(2'')-Ia	-	1..439744	439744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (10-167)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19052.57 Da
• Isoelectric Point: 4.5535

>T124721 WP_019437783.1 NZ_CP039991:c148341-147817 [Pseudomonas aeruginosa]
MKIIGVQDLPCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQASAFLPPGLLISYSLSLDRVI
DFTGGYNDTWAPIWQDFFCDWRKIRFNDGVEPPSWIIGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP

Nucleotide

• Length: 525 bp

>T124721 NZ_CP039991:c148341-147817 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTTGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCTCCTGGTCTGCTGATCAGCTATTCCCTCAGTCTGGATCGAGTGATC
GACTTCACCGGCGGCTATAACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAGCCGCCGAGCTGGATTATCGGAGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGACGCGCCCGTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14753.87 Da
• Isoelectric Point: 7.2048

>AT124721 WP_019437782.1 NZ_CP039991:c148733-148338 [Pseudomonas aeruginosa]
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFGYKTAEEVVSEGKTEQLIAFLQSWEAGAQG

Nucleotide

• Length: 396 bp

>AT124721 NZ_CP039991:c148733-148338 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCTCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGCGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCGGCTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCTTGGGAAGCTGGTGCCCAAGGATGA

Structures

Toxin

• AlphaFold DB: A0A2L1KI32

Antitoxin

• AlphaFold DB: A0A2L1KH13