Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4151206..4151825 Replicon chromosome
Accession NZ_CP039960
Organism Klebsiella pneumoniae strain EAN40

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FC649_RS20600 Protein ID WP_002892050.1
Coordinates 4151607..4151825 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FC649_RS20595 Protein ID WP_002892066.1
Coordinates 4151206..4151580 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC649_RS20585 4146358..4147551 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FC649_RS20590 4147574..4150720 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
FC649_RS20595 4151206..4151580 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FC649_RS20600 4151607..4151825 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FC649_RS20605 4151984..4152550 + 567 WP_002892030.1 maltose O-acetyltransferase -
FC649_RS20610 4152522..4152662 - 141 WP_004147370.1 hypothetical protein -
FC649_RS20615 4152683..4153153 + 471 WP_002892026.1 YlaC family protein -
FC649_RS20620 4153128..4154579 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FC649_RS20625 4154680..4155378 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
FC649_RS20630 4155375..4155515 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FC649_RS20635 4155515..4155778 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T124703 WP_002892050.1 NZ_CP039960:4151607-4151825 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T124703 NZ_CP039960:4151607-4151825 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT124703 WP_002892066.1 NZ_CP039960:4151206-4151580 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT124703 NZ_CP039960:4151206-4151580 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References