Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374099..5374724 Replicon chromosome
Accession NZ_CP039957
Organism Klebsiella pneumoniae strain EAN23

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FC648_RS26430 Protein ID WP_002882817.1
Coordinates 5374099..5374482 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FC648_RS26435 Protein ID WP_004150355.1
Coordinates 5374482..5374724 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC648_RS26415 5371465..5372367 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FC648_RS26420 5372364..5372999 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FC648_RS26425 5372996..5373925 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FC648_RS26430 5374099..5374482 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FC648_RS26435 5374482..5374724 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FC648_RS26440 5374929..5375846 + 918 WP_002882812.1 alpha/beta hydrolase -
FC648_RS26445 5375860..5376801 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FC648_RS26450 5376846..5377283 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FC648_RS26455 5377280..5378140 - 861 WP_002882807.1 virulence factor BrkB family protein -
FC648_RS26460 5378134..5378733 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124687 WP_002882817.1 NZ_CP039957:c5374482-5374099 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124687 NZ_CP039957:c5374482-5374099 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124687 WP_004150355.1 NZ_CP039957:c5374724-5374482 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124687 NZ_CP039957:c5374724-5374482 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References