Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5321078..5321703 Replicon chromosome
Accession NZ_CP039954
Organism Klebsiella pneumoniae strain AR142_2b

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FC647_RS25755 Protein ID WP_002882817.1
Coordinates 5321078..5321461 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FC647_RS25760 Protein ID WP_004150355.1
Coordinates 5321461..5321703 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC647_RS25740 5318444..5319346 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FC647_RS25745 5319343..5319978 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FC647_RS25750 5319975..5320904 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FC647_RS25755 5321078..5321461 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FC647_RS25760 5321461..5321703 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FC647_RS25765 5321908..5322825 + 918 WP_009484979.1 alpha/beta hydrolase -
FC647_RS25770 5322839..5323780 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FC647_RS25775 5323825..5324262 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FC647_RS25780 5324259..5325119 - 861 WP_004146232.1 virulence factor BrkB family protein -
FC647_RS25785 5325113..5325712 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124666 WP_002882817.1 NZ_CP039954:c5321461-5321078 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124666 NZ_CP039954:c5321461-5321078 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124666 WP_004150355.1 NZ_CP039954:c5321703-5321461 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124666 NZ_CP039954:c5321703-5321461 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References