Detailed information of TA system TA124663

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4743917..4744620
• Replicon: chromosome
• Accession: NZ_CP039954
• Organism: Klebsiella pneumoniae strain AR142_2b

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: FC647_RS23060
• Protein ID: WP_071994632.1
• Coordinates: 4743917..4744258 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: FC647_RS23065
• Protein ID: WP_032434296.1
• Coordinates: 4744279..4744620 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FC647_RS23050	4740232..4741101	+	870	WP_023317468.1	HNH endonuclease	-
FC647_RS23055	4741692..4743725	+	2034	WP_050598589.1	hypothetical protein	-
FC647_RS23060	4743917..4744258	-	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
FC647_RS23065	4744279..4744620	-	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FC647_RS23070	4744631..4745173	-	543	WP_032434298.1	DNA repair protein RadC	-
FC647_RS23075	4745186..4745626	-	441	WP_032434300.1	antirestriction protein	-
FC647_RS23080	4745657..4746478	-	822	WP_032434301.1	DUF945 domain-containing protein	-
FC647_RS23085	4746598..4747071	-	474	WP_032434303.1	hypothetical protein	-
FC647_RS23090	4747143..4747595	-	453	WP_032410767.1	hypothetical protein	-
FC647_RS23095	4747631..4748347	-	717	WP_032434305.1	WYL domain-containing protein	-
FC647_RS23100	4748591..4749465	-	875	Protein_4525	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4732214..4777887	45673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T124663 WP_071994632.1 NZ_CP039954:c4744258-4743917 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T124663 NZ_CP039954:c4744258-4743917 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT124663 WP_032434296.1 NZ_CP039954:c4744620-4744279 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT124663 NZ_CP039954:c4744620-4744279 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA