Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2065953..2066578 Replicon chromosome
Accession NZ_CP039951
Organism Klebsiella pneumoniae strain 18KM2445b

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FC646_RS09770 Protein ID WP_002882817.1
Coordinates 2065953..2066336 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FC646_RS09775 Protein ID WP_004150355.1
Coordinates 2066336..2066578 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC646_RS09755 2063319..2064221 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FC646_RS09760 2064218..2064853 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FC646_RS09765 2064850..2065779 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FC646_RS09770 2065953..2066336 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FC646_RS09775 2066336..2066578 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FC646_RS09780 2066783..2067700 + 918 WP_009484979.1 alpha/beta hydrolase -
FC646_RS09785 2067714..2068655 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FC646_RS09790 2068700..2069137 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FC646_RS09795 2069134..2069994 - 861 WP_004146232.1 virulence factor BrkB family protein -
FC646_RS09800 2069988..2070587 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124633 WP_002882817.1 NZ_CP039951:c2066336-2065953 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124633 NZ_CP039951:c2066336-2065953 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124633 WP_004150355.1 NZ_CP039951:c2066578-2066336 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124633 NZ_CP039951:c2066578-2066336 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References