Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374528..5375153 Replicon chromosome
Accession NZ_CP039948
Organism Klebsiella pneumoniae strain 19KM57

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FC645_RS26435 Protein ID WP_002882817.1
Coordinates 5374528..5374911 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FC645_RS26440 Protein ID WP_004150355.1
Coordinates 5374911..5375153 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FC645_RS26420 5371894..5372796 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FC645_RS26425 5372793..5373428 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FC645_RS26430 5373425..5374354 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FC645_RS26435 5374528..5374911 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FC645_RS26440 5374911..5375153 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FC645_RS26445 5375358..5376275 + 918 WP_002882812.1 alpha/beta hydrolase -
FC645_RS26450 5376289..5377230 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FC645_RS26455 5377275..5377712 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FC645_RS26460 5377709..5378569 - 861 WP_002882807.1 virulence factor BrkB family protein -
FC645_RS26465 5378563..5379162 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T124626 WP_002882817.1 NZ_CP039948:c5374911-5374528 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T124626 NZ_CP039948:c5374911-5374528 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT124626 WP_004150355.1 NZ_CP039948:c5375153-5374911 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT124626 NZ_CP039948:c5375153-5374911 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References