Detailed information of TA system TA124509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 384987..385473
• Replicon: chromosome
• Accession: NZ_CP039894
• Organism: Rhizobium pusense strain CFBP5875

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: CFBP5875_RS01895
• Protein ID: WP_137068387.1
• Coordinates: 384987..385256 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A1S7Q717
• Locus tag: CFBP5875_RS01900
• Protein ID: WP_004440186.1
• Coordinates: 385240..385473 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBP5875_RS01860	380087..380716	+	630	WP_004440176.1	DNA-3-methyladenine glycosylase I	-
CFBP5875_RS01865	380713..381156	+	444	WP_004440178.1	hypothetical protein	-
CFBP5875_RS01870	381171..382214	-	1044	WP_137068384.1	YeiH family protein	-
CFBP5875_RS01875	382296..383213	+	918	WP_004440182.1	LysR family transcriptional regulator	-
CFBP5875_RS01880	383359..383733	-	375	WP_137068385.1	DUF305 domain-containing protein	-
CFBP5875_RS01885	383791..384150	-	360	WP_137069182.1	hypothetical protein	-
CFBP5875_RS01890	384216..384980	-	765	WP_137068386.1	hypothetical protein	-
CFBP5875_RS01895	384987..385256	-	270	WP_137068387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFBP5875_RS01900	385240..385473	-	234	WP_004440186.1	ribbon-helix-helix protein, CopG family	Antitoxin
CFBP5875_RS01905	385708..387231	+	1524	WP_137068388.1	histidine--tRNA ligase	-
CFBP5875_RS01910	387236..387604	+	369	WP_137068389.1	VOC family protein	-
CFBP5875_RS01915	387621..388745	+	1125	WP_004440189.1	ATP phosphoribosyltransferase regulatory subunit	-
CFBP5875_RS01920	388742..389434	+	693	WP_045532643.1	ATP phosphoribosyltransferase	-
CFBP5875_RS01925	389497..389943	-	447	WP_004440191.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10575.22 Da
• Isoelectric Point: 10.2765

>T124509 WP_137068387.1 NZ_CP039894:c385256-384987 [Rhizobium pusense]
MIWKIEFQHRAVKQLEKLAKQDANRIVSFLADRVVQNENPRRTGAALQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T124509 NZ_CP039894:c385256-384987 [Rhizobium pusense]
TTGATCTGGAAAATTGAGTTTCAGCACCGCGCCGTCAAGCAGCTTGAAAAGCTGGCGAAGCAAGATGCGAACCGCATCGT
TTCGTTTCTGGCAGACAGGGTGGTGCAAAATGAAAATCCCCGCAGAACCGGTGCCGCCCTGCAAGGTTCCGAACTTGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTATCGCATCATCTGCGATATACAGGATCATAAGCTCGTCGTGCTGGTGGTCGAA
ATCGGCCATCGCCGCGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9225.31 Da
• Isoelectric Point: 4.7359

>AT124509 WP_004440186.1 NZ_CP039894:c385473-385240 [Rhizobium pusense]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRRIANNERTYTLEEVMRNLDLEN

Nucleotide

• Length: 234 bp

>AT124509 NZ_CP039894:c385473-385240 [Rhizobium pusense]
ATGAGCAAACAGACGGCCATCCGCCTCCCGGATGAGACCTATGAGCGGCTGAAAGCGCTTTCCGAGCGCACCGGACGCAC
ATCTGCCTATTACATTCGCGAGGCGATCGAGAAGCATATCGAAGATATGGAAGATCTTTATCTCGCCGAAGAGGCGACGC
GGCGACGGATCGCAAACAATGAGCGCACCTATACTCTGGAAGAAGTGATGCGCAACCTTGATCTGGAAAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S7Q717