Detailed information of TA system TA124490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3070618..3071222
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: FCU27_RS15180
• Protein ID: WP_003414492.1
• Coordinates: 3070618..3071010 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: FCU27_RS15185
• Protein ID: WP_003414495.1
• Coordinates: 3071007..3071222 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS15150	3065768..3066556	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FCU27_RS15155	3066890..3067435	-	546	WP_003904931.1	DUF1802 family protein	-
FCU27_RS15160	3067707..3068591	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FCU27_RS15165	3068594..3069481	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
FCU27_RS15170	3069786..3070331	-	546	WP_003899500.1	DUF1802 family protein	-
FCU27_RS15175	3070328..3070597	-	270	WP_003414489.1	DUF2277 family protein	-
FCU27_RS15180	3070618..3071010	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
FCU27_RS15185	3071007..3071222	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FCU27_RS15190	3071269..3072018	+	750	WP_003414497.1	enoyl-CoA hydratase	-
FCU27_RS15195	3072097..3073179	-	1083	WP_011799293.1	ABC transporter ATP-binding protein	-
FCU27_RS15200	3073172..3074482	-	1311	Protein_2842	ABC transporter substrate-binding protein	-
FCU27_RS15205	3074485..3075312	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
FCU27_RS15210	3075309..3076220	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T124490 WP_003414492.1 NZ_CP039851:c3071010-3070618 [Mycobacterium tuberculosis variant bovis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T124490 NZ_CP039851:c3071010-3070618 [Mycobacterium tuberculosis variant bovis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT124490 WP_003414495.1 NZ_CP039851:c3071222-3071007 [Mycobacterium tuberculosis variant bovis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT124490 NZ_CP039851:c3071222-3071007 [Mycobacterium tuberculosis variant bovis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8