Detailed information of TA system TA124488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3043627..3044197
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FCU27_RS15035
• Protein ID: WP_003414166.1
• Coordinates: 3043627..3043983 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FCU27_RS15040
• Protein ID: WP_003901465.1
• Coordinates: 3043967..3044197 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS15015	3039079..3040767	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FCU27_RS15020	3040771..3041097	-	327	WP_003414157.1	hypothetical protein	-
FCU27_RS15025	3041270..3041857	+	588	WP_003914429.1	DUF3558 family protein	-
FCU27_RS15030	3041876..3043525	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FCU27_RS15035	3043627..3043983	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FCU27_RS15040	3043967..3044197	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FCU27_RS15045	3044240..3045283	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FCU27_RS15050	3045474..3045749	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
FCU27_RS21400	3045925..3046179	-	255	WP_003917684.1	hypothetical protein	-
FCU27_RS15060	3046327..3046731	+	405	WP_003414181.1	hypothetical protein	-
FCU27_RS15065	3046728..3046919	+	192	WP_003414184.1	hypothetical protein	-
FCU27_RS15070	3046983..3048272	+	1290	Protein_2816	transposase family protein	-
FCU27_RS15075	3048506..3048763	+	258	WP_003899489.1	hypothetical protein	-
FCU27_RS15080	3048868..3049179	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T124488 WP_003414166.1 NZ_CP039851:c3043983-3043627 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T124488 NZ_CP039851:c3043983-3043627 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT124488 WP_003901465.1 NZ_CP039851:c3044197-3043967 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT124488 NZ_CP039851:c3044197-3043967 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3