Detailed information of TA system TA124486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3003631..3004310
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FCU27_RS14805
• Protein ID: WP_003414059.1
• Coordinates: 3003631..3004047 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FCU27_RS14810
• Protein ID: WP_003414061.1
• Coordinates: 3004044..3004310 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS14785	2999683..3000585	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FCU27_RS14790	3000654..3001406	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FCU27_RS14795	3001650..3001925	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FCU27_RS14800	3001922..3003544	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FCU27_RS14805	3003631..3004047	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FCU27_RS14810	3004044..3004310	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FCU27_RS14815	3004336..3004731	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FCU27_RS14820	3004728..3004997	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FCU27_RS14825	3005007..3006101	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FCU27_RS14830	3006098..3006517	-	420	Protein_2769	winged helix-turn-helix domain-containing protein	-
FCU27_RS14840	3006591..3007070	-	480	WP_003414073.1	dihydrofolate reductase	-
FCU27_RS14845	3007141..3007941	-	801	WP_003911953.1	thymidylate synthase	-
FCU27_RS14850	3008097..3008834	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T124486 WP_003414059.1 NZ_CP039851:c3004047-3003631 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T124486 NZ_CP039851:c3004047-3003631 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT124486 WP_003414061.1 NZ_CP039851:c3004310-3004044 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT124486 NZ_CP039851:c3004310-3004044 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64