Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2813176..2813807 Replicon chromosome
Accession NZ_CP039851
Organism Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag FCU27_RS13725 Protein ID WP_003413174.1
Coordinates 2813430..2813807 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag FCU27_RS13720 Protein ID WP_003413167.1
Coordinates 2813176..2813433 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCU27_RS13680 2808434..2808748 + 315 WP_009937839.1 hypothetical protein -
FCU27_RS13685 2809044..2810255 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FCU27_RS13695 2810382..2811041 + 660 WP_003900846.1 LppA family lipoprotein -
FCU27_RS13700 2811038..2811697 + 660 WP_003900847.1 hypothetical protein -
FCU27_RS13705 2811694..2812356 + 663 WP_003900848.1 hypothetical protein -
FCU27_RS13710 2812353..2812631 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FCU27_RS13715 2812724..2813137 + 414 WP_003413164.1 PIN domain nuclease -
FCU27_RS13720 2813176..2813433 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
FCU27_RS13725 2813430..2813807 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
FCU27_RS13730 2813823..2814197 - 375 WP_003413177.1 hypothetical protein -
FCU27_RS13735 2814297..2814692 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
FCU27_RS13740 2814689..2814934 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
FCU27_RS13750 2815345..2815764 - 420 WP_003413190.1 A24 family peptidase -
FCU27_RS13755 2815776..2816603 - 828 WP_010950736.1 shikimate dehydrogenase -
FCU27_RS13760 2816581..2817834 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FCU27_RS13765 2817827..2818339 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T124483 WP_003413174.1 NZ_CP039851:2813430-2813807 [Mycobacterium tuberculosis variant bovis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T124483 NZ_CP039851:2813430-2813807 [Mycobacterium tuberculosis variant bovis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT124483 WP_003413167.1 NZ_CP039851:2813176-2813433 [Mycobacterium tuberculosis variant bovis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT124483 NZ_CP039851:2813176-2813433 [Mycobacterium tuberculosis variant bovis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References