Detailed information of TA system TA124470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2177056..2177759
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FCU27_RS10520
• Protein ID: WP_003409778.1
• Coordinates: 2177056..2177385 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FCU27_RS10525
• Protein ID: WP_003409780.1
• Coordinates: 2177382..2177759 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS10500	2173439..2174509	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FCU27_RS10505	2174506..2175021	+	516	WP_003409718.1	flavin reductase family protein	-
FCU27_RS10510	2175018..2176079	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FCU27_RS10515	2176076..2176846	+	771	WP_003409775.1	SDR family oxidoreductase	-
FCU27_RS10520	2177056..2177385	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FCU27_RS10525	2177382..2177759	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FCU27_RS10530	2177756..2178346	-	591	WP_003910477.1	SEC-C domain-containing protein	-
FCU27_RS10535	2178401..2179765	+	1365	WP_011799233.1	HNH endonuclease	-
FCU27_RS10540	2179920..2180372	-	453	WP_010950636.1	lipoprotein	-
FCU27_RS10545	2180436..2180837	+	402	WP_003409869.1	hypothetical protein	-
FCU27_RS10550	2180830..2181012	-	183	WP_003409870.1	hypothetical protein	-
FCU27_RS10555	2181126..2181476	-	351	WP_003409871.1	hypothetical protein	-
FCU27_RS10560	2181487..2182389	-	903	WP_003409874.1	hypothetical protein	-
FCU27_RS10565	2182410..2182601	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T124470 WP_003409778.1 NZ_CP039851:c2177385-2177056 [Mycobacterium tuberculosis variant bovis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T124470 NZ_CP039851:c2177385-2177056 [Mycobacterium tuberculosis variant bovis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT124470 WP_003409780.1 NZ_CP039851:c2177759-2177382 [Mycobacterium tuberculosis variant bovis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT124470 NZ_CP039851:c2177759-2177382 [Mycobacterium tuberculosis variant bovis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8