Detailed information of TA system TA124461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241278..1241846
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FCU27_RS06185
• Protein ID: WP_003405865.1
• Coordinates: 1241472..1241846 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FCU27_RS06180
• Protein ID: WP_003405863.1
• Coordinates: 1241278..1241475 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS06160	1237319..1237957	-	639	WP_011799158.1	lipid droplet-associated protein	-
FCU27_RS06165	1238047..1239054	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FCU27_RS06170	1239071..1240054	-	984	WP_003898731.1	hypothetical protein	-
FCU27_RS06175	1240117..1241190	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
FCU27_RS06180	1241278..1241475	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FCU27_RS06185	1241472..1241846	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FCU27_RS06190	1242049..1242747	+	699	WP_010950494.1	hypothetical protein	-
FCU27_RS06195	1242865..1243050	+	186	WP_003901093.1	hypothetical protein	-
FCU27_RS06200	1242977..1243252	-	276	WP_003405867.1	hypothetical protein	-
FCU27_RS06205	1243495..1243818	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FCU27_RS06210	1243833..1244543	-	711	WP_003911399.1	hypothetical protein	-
FCU27_RS21255	1244565..1244774	+	210	WP_003911400.1	hypothetical protein	-
FCU27_RS06215	1244726..1245433	-	708	Protein_1158	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T124461 WP_003405865.1 NZ_CP039851:1241472-1241846 [Mycobacterium tuberculosis variant bovis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T124461 NZ_CP039851:1241472-1241846 [Mycobacterium tuberculosis variant bovis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT124461 WP_003405863.1 NZ_CP039851:1241278-1241475 [Mycobacterium tuberculosis variant bovis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT124461 NZ_CP039851:1241278-1241475 [Mycobacterium tuberculosis variant bovis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07