Detailed information of TA system TA124448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704587..705230
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: FCU27_RS03300
• Protein ID: WP_003403187.1
• Coordinates: 704829..705230 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: FCU27_RS03295
• Protein ID: WP_003403184.1
• Coordinates: 704587..704832 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS03255	699867..700397	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FCU27_RS03260	700381..701142	-	762	WP_010950420.1	two-component system response regulator TcrA	-
FCU27_RS03265	701199..701510	+	312	WP_003403164.1	hypothetical protein	-
FCU27_RS03270	701582..702532	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
FCU27_RS03280	702773..703357	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FCU27_RS03285	703359..704069	+	711	Protein_627	transposase	-
FCU27_RS03290	704072..704542	+	471	WP_003898523.1	hypothetical protein	-
FCU27_RS03295	704587..704832	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FCU27_RS03300	704829..705230	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
FCU27_RS03315	705618..705785	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
FCU27_RS03320	705818..706015	-	198	WP_003403191.1	hypothetical protein	-
FCU27_RS03325	706095..707252	-	1158	WP_003403193.1	hypothetical protein	-
FCU27_RS03330	707304..707525	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
FCU27_RS03335	707667..708272	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T124448 WP_003403187.1 NZ_CP039851:704829-705230 [Mycobacterium tuberculosis variant bovis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T124448 NZ_CP039851:704829-705230 [Mycobacterium tuberculosis variant bovis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT124448 WP_003403184.1 NZ_CP039851:704587-704832 [Mycobacterium tuberculosis variant bovis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT124448 NZ_CP039851:704587-704832 [Mycobacterium tuberculosis variant bovis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91