Detailed information of TA system TA124446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 696182..696828
• Replicon: chromosome
• Accession: NZ_CP039851
• Organism: Mycobacterium tuberculosis variant bovis strain Danish delta-sapM

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: FCU27_RS03225
• Protein ID: WP_003403122.1
• Coordinates: 696182..696574 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: FCU27_RS03230
• Protein ID: WP_003403125.1
• Coordinates: 696571..696828 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU27_RS03210	691842..693370	+	1529	Protein_613	virulence factor Mce family protein	-
FCU27_RS03215	693433..694575	+	1143	WP_003911253.1	virulence factor Mce family protein	-
FCU27_RS03220	694580..696130	+	1551	WP_003403119.1	MCE family protein	-
FCU27_RS03225	696182..696574	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
FCU27_RS03230	696571..696828	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
FCU27_RS03235	697011..698246	-	1236	WP_003403128.1	ATP-binding protein	-
FCU27_RS03240	698497..698910	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
FCU27_RS03245	698907..699143	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FCU27_RS03250	699247..699753	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FCU27_RS03255	699867..700397	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FCU27_RS03260	700381..701142	-	762	WP_010950420.1	two-component system response regulator TcrA	-
FCU27_RS03265	701199..701510	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T124446 WP_003403122.1 NZ_CP039851:c696574-696182 [Mycobacterium tuberculosis variant bovis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T124446 NZ_CP039851:c696574-696182 [Mycobacterium tuberculosis variant bovis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT124446 WP_003403125.1 NZ_CP039851:c696828-696571 [Mycobacterium tuberculosis variant bovis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT124446 NZ_CP039851:c696828-696571 [Mycobacterium tuberculosis variant bovis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86