Detailed information of TA system TA124428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3043562..3044132
• Replicon: chromosome
• Accession: NZ_CP039850
• Organism: Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: FCU26_RS15035
• Protein ID: WP_003414166.1
• Coordinates: 3043562..3043918 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: FCU26_RS15040
• Protein ID: WP_003901465.1
• Coordinates: 3043902..3044132 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU26_RS15015	3039014..3040702	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
FCU26_RS15020	3040706..3041032	-	327	WP_003414157.1	hypothetical protein	-
FCU26_RS15025	3041205..3041792	+	588	WP_003914429.1	DUF3558 family protein	-
FCU26_RS15030	3041811..3043460	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
FCU26_RS15035	3043562..3043918	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
FCU26_RS15040	3043902..3044132	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
FCU26_RS15045	3044175..3045218	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
FCU26_RS15050	3045409..3045684	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
FCU26_RS21780	3045860..3046114	-	255	WP_003917684.1	hypothetical protein	-
FCU26_RS15060	3046262..3046666	+	405	WP_003414181.1	hypothetical protein	-
FCU26_RS15065	3046663..3046854	+	192	WP_003414184.1	hypothetical protein	-
FCU26_RS15070	3046918..3048207	+	1290	Protein_2815	transposase family protein	-
FCU26_RS15075	3048441..3048698	+	258	WP_003899489.1	hypothetical protein	-
FCU26_RS15080	3048803..3049114	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T124428 WP_003414166.1 NZ_CP039850:c3043918-3043562 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T124428 NZ_CP039850:c3043918-3043562 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT124428 WP_003901465.1 NZ_CP039850:c3044132-3043902 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT124428 NZ_CP039850:c3044132-3043902 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3