Detailed information of TA system TA124426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3003566..3004245
• Replicon: chromosome
• Accession: NZ_CP039850
• Organism: Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FCU26_RS14805
• Protein ID: WP_003414059.1
• Coordinates: 3003566..3003982 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FCU26_RS14810
• Protein ID: WP_003414061.1
• Coordinates: 3003979..3004245 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU26_RS14785	2999618..3000520	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FCU26_RS14790	3000589..3001341	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FCU26_RS14795	3001585..3001860	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FCU26_RS14800	3001857..3003479	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FCU26_RS14805	3003566..3003982	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FCU26_RS14810	3003979..3004245	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FCU26_RS14815	3004271..3004666	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FCU26_RS14820	3004663..3004932	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FCU26_RS14825	3004942..3006036	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FCU26_RS14830	3006033..3006452	-	420	Protein_2768	winged helix-turn-helix domain-containing protein	-
FCU26_RS14840	3006526..3007005	-	480	WP_003414073.1	dihydrofolate reductase	-
FCU26_RS14845	3007076..3007876	-	801	WP_003911953.1	thymidylate synthase	-
FCU26_RS14850	3008032..3008769	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T124426 WP_003414059.1 NZ_CP039850:c3003982-3003566 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T124426 NZ_CP039850:c3003982-3003566 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT124426 WP_003414061.1 NZ_CP039850:c3004245-3003979 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT124426 NZ_CP039850:c3004245-3003979 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64