Detailed information of TA system TA124421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2798165..2798805
• Replicon: chromosome
• Accession: NZ_CP039850
• Organism: Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FCU26_RS13620
• Protein ID: WP_003412970.1
• Coordinates: 2798165..2798584 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FCU26_RS13625
• Protein ID: WP_003412975.1
• Coordinates: 2798581..2798805 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU26_RS13590	2793750..2794472	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FCU26_RS13600	2794989..2795216	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FCU26_RS13605	2795213..2795614	+	402	WP_003412963.1	PIN domain-containing protein	-
FCU26_RS13610	2795649..2796569	-	921	WP_011799273.1	restriction endonuclease	-
FCU26_RS21750	2796910..2797155	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FCU26_RS13615	2797214..2798164	+	951	WP_003911916.1	Lsr2 family protein	-
FCU26_RS13620	2798165..2798584	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FCU26_RS13625	2798581..2798805	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FCU26_RS13630	2798836..2801679	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FCU26_RS13635	2801751..2802152	-	402	WP_003412981.1	hypothetical protein	-
FCU26_RS13640	2802152..2802622	-	471	WP_003412985.1	transcription antitermination factor NusB	-
FCU26_RS13645	2802625..2803188	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T124421 WP_003412970.1 NZ_CP039850:c2798584-2798165 [Mycobacterium tuberculosis variant bovis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T124421 NZ_CP039850:c2798584-2798165 [Mycobacterium tuberculosis variant bovis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT124421 WP_003412975.1 NZ_CP039850:c2798805-2798581 [Mycobacterium tuberculosis variant bovis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT124421 NZ_CP039850:c2798805-2798581 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09