Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2192951..2193537 Replicon chromosome
Accession NZ_CP039850
Organism Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FCU26_RS10680 Protein ID WP_003410010.1
Coordinates 2192951..2193295 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FCU26_RS10685 Protein ID WP_003410014.1
Coordinates 2193289..2193537 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCU26_RS10645 2189840..2190256 + 417 WP_162014414.1 hypothetical protein -
FCU26_RS10650 2190341..2190865 + 525 WP_011799234.1 methyltransferase -
FCU26_RS10660 2191385..2191945 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
FCU26_RS10665 2191942..2192283 - 342 WP_003410003.1 DUF2384 domain-containing protein -
FCU26_RS10670 2192369..2192626 + 258 WP_003410006.1 hypothetical protein -
FCU26_RS10675 2192527..2192862 - 336 WP_003410009.1 dehydrogenase -
FCU26_RS10680 2192951..2193295 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FCU26_RS10685 2193289..2193537 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FCU26_RS10690 2193637..2195952 - 2316 WP_003899120.1 cation transporter ATPase CptG -
FCU26_RS10695 2195949..2196221 - 273 WP_003410017.1 DUF1490 family protein -
FCU26_RS10700 2196274..2196630 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FCU26_RS10705 2196787..2197554 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T124414 WP_003410010.1 NZ_CP039850:c2193295-2192951 [Mycobacterium tuberculosis variant bovis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T124414 NZ_CP039850:c2193295-2192951 [Mycobacterium tuberculosis variant bovis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT124414 WP_003410014.1 NZ_CP039850:c2193537-2193289 [Mycobacterium tuberculosis variant bovis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT124414 NZ_CP039850:c2193537-2193289 [Mycobacterium tuberculosis variant bovis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References