Detailed information of TA system TA124397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015487..1016106
• Replicon: chromosome
• Accession: NZ_CP039850
• Organism: Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FCU26_RS05005
• Protein ID: WP_003404726.1
• Coordinates: 1015672..1016106 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: FCU26_RS05000
• Protein ID: WP_003404724.1
• Coordinates: 1015487..1015666 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCU26_RS04990	1010960..1012540	+	1581	WP_011799139.1	serine hydrolase	-
FCU26_RS04995	1012537..1014930	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FCU26_RS21610	1015203..1015361	+	159	WP_003404720.1	hypothetical protein	-
FCU26_RS05000	1015487..1015666	+	180	WP_003404724.1	antitoxin	Antitoxin
FCU26_RS05005	1015672..1016106	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FCU26_RS05010	1016204..1016977	+	774	WP_003404735.1	VOC family protein	-
FCU26_RS05015	1017042..1017491	+	450	WP_003404738.1	hypothetical protein	-
FCU26_RS05020	1017626..1017856	-	231	WP_003898642.1	hypothetical protein	-
FCU26_RS05025	1018023..1019531	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FCU26_RS05030	1019533..1020771	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T124397 WP_003404726.1 NZ_CP039850:1015672-1016106 [Mycobacterium tuberculosis variant bovis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T124397 NZ_CP039850:1015672-1016106 [Mycobacterium tuberculosis variant bovis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT124397 WP_003404724.1 NZ_CP039850:1015487-1015666 [Mycobacterium tuberculosis variant bovis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT124397 NZ_CP039850:1015487-1015666 [Mycobacterium tuberculosis variant bovis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5