Detailed information of TA system TA124364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 56808..57409
• Replicon: plasmid pUSDA5905_1
• Accession: NZ_CP039843
• Organism: Escherichia coli O157:H7 strain USDA5905

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9Z1Q0
• Locus tag: E3175_RS27410
• Protein ID: WP_001216047.1
• Coordinates: 57029..57409 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: E3175_RS27405
• Protein ID: WP_001190712.1
• Coordinates: 56808..57029 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3175_RS27355	52180..52470	+	291	WP_001018057.1	DUF4752 family protein	-
E3175_RS27360	52467..53180	+	714	WP_000672529.1	hypothetical protein	-
E3175_RS27365	53177..53476	+	300	WP_000215167.1	hypothetical protein	-
E3175_RS27370	53478..54164	+	687	WP_000161573.1	ead/Ea22-like family protein	-
E3175_RS27375	54166..54315	+	150	WP_001151811.1	hypothetical protein	-
E3175_RS27380	54319..55335	+	1017	WP_000208041.1	hypothetical protein	-
E3175_RS27385	55319..55603	+	285	WP_001142394.1	hypothetical protein	-
E3175_RS27390	55588..55797	-	210	WP_001344848.1	hypothetical protein	-
E3175_RS27395	55971..56222	+	252	WP_001283837.1	DNA polymerase III subunit theta	-
E3175_RS27400	56346..56735	+	390	WP_000506726.1	DNA repair protein	-
E3175_RS27405	56808..57029	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3175_RS27410	57029..57409	+	381	WP_001216047.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E3175_RS27415	57414..57593	+	180	WP_000113019.1	hypothetical protein	-
E3175_RS27420	57621..58664	+	1044	WP_000644103.1	DUF968 domain-containing protein	-
E3175_RS27425	58753..59205	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
E3175_RS27430	59292..60485	+	1194	WP_000219604.1	terminase	-
E3175_RS27435	60485..61969	+	1485	WP_000124156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1A	-	1..95647	95647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T124364 WP_001216047.1 NZ_CP039843:57029-57409 [Escherichia coli O157:H7]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T124364 NZ_CP039843:57029-57409 [Escherichia coli O157:H7]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT124364 WP_001190712.1 NZ_CP039843:56808-57029 [Escherichia coli O157:H7]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT124364 NZ_CP039843:56808-57029 [Escherichia coli O157:H7]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: U9Z1Q0

Antitoxin

• AlphaFold DB: A0A829CJB6