Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 26426..26973 Replicon plasmid pUSDA5905_2
Accession NZ_CP039842
Organism Escherichia coli O157:H7 strain USDA5905

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag E3175_RS26825 Protein ID WP_000969997.1
Coordinates 26692..26973 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag E3175_RS26820 Protein ID WP_000079946.1
Coordinates 26426..26695 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3175_RS26780 22127..22642 + 516 WP_000998852.1 type II secretion system protein M -
E3175_RS26785 22694..23527 + 834 WP_000088803.1 A24 family peptidase -
E3175_RS26790 23621..24022 + 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
E3175_RS26795 24097..24312 + 216 Protein_31 IS3 family transposase -
E3175_RS26800 24611..24904 + 294 Protein_32 hypothetical protein -
E3175_RS26805 25133..25381 + 249 WP_000083841.1 replication regulatory protein RepA -
E3175_RS26810 25404..25511 + 108 Protein_34 incFII family plasmid replication initiator RepA -
E3175_RS26815 26213..26371 + 159 WP_000829588.1 hypothetical protein -
E3175_RS26820 26426..26695 + 270 WP_000079946.1 hypothetical protein Antitoxin
E3175_RS26825 26692..26973 + 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3175_RS26830 27169..27510 - 342 WP_000578011.1 hypothetical protein -
E3175_RS26835 27810..28397 + 588 WP_001418189.1 serine acetyltransferase -
E3175_RS26840 28679..29272 - 594 WP_000517696.1 hypothetical protein -
E3175_RS26845 29625..30971 + 1347 WP_001132032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - stcE / exeE / exeG / nleA/espI 1..62787 62787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T124362 WP_000969997.1 NZ_CP039842:26692-26973 [Escherichia coli O157:H7]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T124362 NZ_CP039842:26692-26973 [Escherichia coli O157:H7]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT124362 WP_000079946.1 NZ_CP039842:26426-26695 [Escherichia coli O157:H7]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT124362 NZ_CP039842:26426-26695 [Escherichia coli O157:H7]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References