Detailed information of TA system TA124352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2638050..2638576
• Replicon: chromosome
• Accession: NZ_CP039837
• Organism: Escherichia coli O157:H7 strain USDA5905

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: E3175_RS13105
• Protein ID: WP_000323025.1
• Coordinates: 2638289..2638576 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: E3175_RS13100
• Protein ID: WP_000534858.1
• Coordinates: 2638050..2638289 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3175_RS13075	2633412..2633768	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
E3175_RS13080	2635075..2635257	-	183	WP_000955178.1	hypothetical protein	-
E3175_RS13085	2635435..2636748	+	1314	WP_000589005.1	ISNCY family transposase	-
E3175_RS13090	2637185..2637517	-	333	WP_001301033.1	protein FlxA	-
E3175_RS13095	2637720..2638025	-	306	WP_001326990.1	hypothetical protein	-
E3175_RS13100	2638050..2638289	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
E3175_RS13105	2638289..2638576	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
E3175_RS13110	2638648..2638803	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
E3175_RS13115	2639020..2639271	+	252	WP_000980994.1	hypothetical protein	-
E3175_RS13120	2639338..2639616	+	279	WP_012304870.1	hypothetical protein	-
E3175_RS13125	2639618..2640667	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
E3175_RS13130	2640681..2641433	+	753	WP_001047135.1	antitermination protein	-
E3175_RS13135	2641711..2641800	-	90	WP_120795389.1	hypothetical protein	-
E3175_RS13140	2641855..2642067	-	213	WP_000087756.1	cold shock-like protein CspF	-
E3175_RS13145	2642368..2642583	+	216	WP_000066484.1	cold shock-like protein CspB	-
E3175_RS13150	2643337..2643552	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2620897..2673626	52729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T124352 WP_000323025.1 NZ_CP039837:2638289-2638576 [Escherichia coli O157:H7]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T124352 NZ_CP039837:2638289-2638576 [Escherichia coli O157:H7]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT124352 WP_000534858.1 NZ_CP039837:2638050-2638289 [Escherichia coli O157:H7]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT124352 NZ_CP039837:2638050-2638289 [Escherichia coli O157:H7]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8