Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52292..52817 Replicon plasmid pMB41_1
Accession NZ_CP039835
Organism Escherichia coli O157:H7 strain MB41-1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FCV42_RS27600 Protein ID WP_001159868.1
Coordinates 52512..52817 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FCV42_RS27595 Protein ID WP_000813634.1
Coordinates 52292..52510 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCV42_RS27565 47558..47788 + 231 WP_010891288.1 hypothetical protein -
FCV42_RS27570 47909..48649 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
FCV42_RS27575 48934..49912 - 979 Protein_48 replication initiation protein -
FCV42_RS27580 50338..50538 - 201 WP_000708307.1 hypothetical protein -
FCV42_RS27585 50535..51155 - 621 Protein_50 hypothetical protein -
FCV42_RS27590 51152..51834 - 683 Protein_51 DNA-binding protein -
FCV42_RS27595 52292..52510 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FCV42_RS27600 52512..52817 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FCV42_RS27605 52818..53625 + 808 Protein_54 phage integrase family protein -
FCV42_RS27615 55523..55861 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
FCV42_RS27620 56449..57614 + 1166 Protein_57 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyA / hlyA / hlyB / hlyB / hlyD / toxB / toxB / toxB 1..93704 93704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T124335 WP_001159868.1 NZ_CP039835:52512-52817 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T124335 NZ_CP039835:52512-52817 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT124335 WP_000813634.1 NZ_CP039835:52292-52510 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT124335 NZ_CP039835:52292-52510 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References