Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94621..95264 Replicon plasmid pC2414-2-KPC
Accession NZ_CP039820
Organism Klebsiella pneumoniae strain C2414

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FA958_RS28760 Protein ID WP_001044770.1
Coordinates 94621..95037 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FA958_RS28765 Protein ID WP_001261282.1
Coordinates 95034..95264 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FA958_RS28735 90724..90996 - 273 Protein_112 transposase -
FA958_RS28740 91025..91722 - 698 Protein_113 IS1 family transposase -
FA958_RS28750 91978..93000 - 1023 WP_000361404.1 helicase UvrD -
FA958_RS28755 92985..94547 - 1563 WP_004206609.1 AAA family ATPase -
FA958_RS28760 94621..95037 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FA958_RS28765 95034..95264 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FA958_RS28770 95221..95682 + 462 WP_014343465.1 hypothetical protein -
FA958_RS28780 95843..96787 + 945 WP_011977810.1 hypothetical protein -
FA958_RS28785 96824..97216 + 393 WP_011977811.1 hypothetical protein -
FA958_RS28790 97274..97795 + 522 WP_094960442.1 hypothetical protein -
FA958_RS28795 97841..98044 + 204 WP_011977813.1 hypothetical protein -
FA958_RS28800 98074..99078 + 1005 WP_011977814.1 hypothetical protein -
FA958_RS28810 99262..100041 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..172001 172001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T124300 WP_001044770.1 NZ_CP039820:c95037-94621 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T124300 NZ_CP039820:c95037-94621 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT124300 WP_001261282.1 NZ_CP039820:c95264-95034 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT124300 NZ_CP039820:c95264-95034 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References