Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 139248..139891 Replicon plasmid pC2660-3-KPC
Accession NZ_CP039810
Organism Klebsiella pneumoniae strain C2660

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FA957_RS29775 Protein ID WP_001044770.1
Coordinates 139475..139891 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FA957_RS29770 Protein ID WP_001261282.1
Coordinates 139248..139478 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FA957_RS29725 134471..135250 - 780 WP_013214009.1 site-specific integrase -
FA957_RS29735 135434..136438 - 1005 WP_011977814.1 hypothetical protein -
FA957_RS29740 136468..136671 - 204 WP_011977813.1 hypothetical protein -
FA957_RS29745 136717..137238 - 522 WP_013214008.1 hypothetical protein -
FA957_RS29750 137296..137688 - 393 WP_011977811.1 hypothetical protein -
FA957_RS29755 137725..138669 - 945 WP_011977810.1 hypothetical protein -
FA957_RS29765 138830..139291 - 462 WP_014343465.1 hypothetical protein -
FA957_RS29770 139248..139478 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FA957_RS29775 139475..139891 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FA957_RS29780 139965..141527 + 1563 WP_004206609.1 AAA family ATPase -
FA957_RS29785 141512..142534 + 1023 WP_000361404.1 helicase UvrD -
FA957_RS29795 142790..143487 + 698 Protein_183 IS1 family transposase -
FA957_RS29800 143516..143788 + 273 Protein_184 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..153556 153556


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T124274 WP_001044770.1 NZ_CP039810:139475-139891 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T124274 NZ_CP039810:139475-139891 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT124274 WP_001261282.1 NZ_CP039810:139248-139478 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT124274 NZ_CP039810:139248-139478 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References