Detailed information of TA system TA124268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 158987..159732
• Replicon: plasmid pC2660-2
• Accession: NZ_CP039809
• Organism: Klebsiella pneumoniae strain C2660

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: FA957_RS28515
• Protein ID: WP_032408901.1
• Coordinates: 159241..159732 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FA957_RS28510
• Protein ID: WP_014386183.1
• Coordinates: 158987..159253 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA957_RS28450	154539..154952	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
FA957_RS28455	154953..155231	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
FA957_RS28460	155221..155541	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FA957_RS28465	155622..155846	-	225	WP_014386189.1	hypothetical protein	-
FA957_RS28470	155857..156069	-	213	WP_014386188.1	hypothetical protein	-
FA957_RS28475	156131..156457	-	327	WP_014386187.1	hypothetical protein	-
FA957_RS28490	157094..157444	-	351	WP_014386186.1	hypothetical protein	-
FA957_RS28495	157441..157713	-	273	WP_032408902.1	hypothetical protein	-
FA957_RS28500	157903..158388	+	486	WP_014386185.1	hypothetical protein	-
FA957_RS28505	158632..158790	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
FA957_RS28510	158987..159253	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
FA957_RS28515	159241..159732	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
FA957_RS28520	160173..160424	-	252	WP_032408900.1	aldo/keto reductase	-
FA957_RS28525	160621..162213	-	1593	Protein_176	IS66 family transposase	-
FA957_RS28530	162244..162594	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
FA957_RS28535	162591..163031	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
FA957_RS28540	163293..164048	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205652	205652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T124268 WP_032408901.1 NZ_CP039809:159241-159732 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T124268 NZ_CP039809:159241-159732 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT124268 WP_014386183.1 NZ_CP039809:158987-159253 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT124268 NZ_CP039809:158987-159253 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6