Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4220999..4221618 Replicon chromosome
Accession NZ_CP039808
Organism Klebsiella pneumoniae strain C2660

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FA957_RS21360 Protein ID WP_002892050.1
Coordinates 4221400..4221618 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FA957_RS21355 Protein ID WP_002892066.1
Coordinates 4220999..4221373 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FA957_RS21345 4216151..4217344 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FA957_RS21350 4217367..4220513 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
FA957_RS21355 4220999..4221373 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FA957_RS21360 4221400..4221618 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FA957_RS21365 4221777..4222343 + 567 WP_002892030.1 maltose O-acetyltransferase -
FA957_RS21370 4222315..4222455 - 141 WP_004147370.1 hypothetical protein -
FA957_RS21375 4222476..4222946 + 471 WP_002892026.1 YlaC family protein -
FA957_RS21380 4222921..4224372 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FA957_RS21385 4224473..4225171 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
FA957_RS21390 4225168..4225308 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FA957_RS21395 4225308..4225571 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T124262 WP_002892050.1 NZ_CP039808:4221400-4221618 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T124262 NZ_CP039808:4221400-4221618 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT124262 WP_002892066.1 NZ_CP039808:4220999-4221373 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT124262 NZ_CP039808:4220999-4221373 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References