Detailed information of TA system TA124247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 70771..71507
• Replicon: plasmid pC3001-3
• Accession: NZ_CP039793
• Organism: Klebsiella quasipneumoniae strain C3001

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FA956_RS28215
• Protein ID: WP_205847347.1
• Coordinates: 70771..71253 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FA956_RS28220
• Protein ID: WP_003026799.1
• Coordinates: 71241..71507 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA956_RS28195	66066..66770	-	705	WP_205847349.1	TIR domain-containing protein	-
FA956_RS28200	66930..68276	-	1347	WP_077251107.1	ISNCY family transposase	-
FA956_RS28205	68506..69138	+	633	WP_001567369.1	hypothetical protein	-
FA956_RS28210	69167..70570	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
FA956_RS28215	70771..71253	-	483	WP_205847347.1	GNAT family N-acetyltransferase	Toxin
FA956_RS28220	71241..71507	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FA956_RS28225	71952..72182	-	231	WP_004206660.1	hypothetical protein	-
FA956_RS28230	72196..72399	-	204	WP_004206661.1	hemolysin expression modulator Hha	-
FA956_RS28235	72460..72951	-	492	WP_004206662.1	hypothetical protein	-
FA956_RS28240	73085..74296	-	1212	WP_032415728.1	hypothetical protein	-
FA956_RS28245	74293..74541	-	249	WP_004193994.1	hypothetical protein	-
FA956_RS28250	74899..75249	+	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FA956_RS28255	75301..75663	+	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / tet(D) / catA2 / aac(3)-IId / blaTEM-1B / blaLAP-2 / qnrS1 / aph(6)-Id / aph(3'')-Ib / sul2	pla	1..206406	206406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17380.01 Da
• Isoelectric Point: 9.5014

>T124247 WP_205847347.1 NZ_CP039793:c71253-70771 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLYGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T124247 NZ_CP039793:c71253-70771 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGTACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT124247 WP_003026799.1 NZ_CP039793:c71507-71241 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT124247 NZ_CP039793:c71507-71241 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66