Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3640263..3640905 Replicon chromosome
Accession NZ_CP039721
Organism Bacillus thuringiensis strain BT-59

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FBF59_RS18665 Protein ID WP_000635965.1
Coordinates 3640263..3640613 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FBF59_RS18670 Protein ID WP_000004570.1
Coordinates 3640618..3640905 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FBF59_RS18650 3637199..3637657 - 459 WP_000343552.1 SprT family protein -
FBF59_RS18655 3637853..3637969 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
FBF59_RS18660 3638027..3640195 - 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
FBF59_RS18665 3640263..3640613 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FBF59_RS18670 3640618..3640905 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FBF59_RS18675 3641214..3642383 - 1170 WP_000390594.1 alanine racemase -
FBF59_RS18680 3642501..3643451 - 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
FBF59_RS18685 3643608..3643967 - 360 WP_000583420.1 holo-ACP synthase -
FBF59_RS18690 3644061..3644633 + 573 WP_000908528.1 rhomboid family intramembrane serine protease -
FBF59_RS18695 3644626..3645588 - 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T124229 WP_000635965.1 NZ_CP039721:c3640613-3640263 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T124229 NZ_CP039721:c3640613-3640263 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT124229 WP_000004570.1 NZ_CP039721:c3640905-3640618 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT124229 NZ_CP039721:c3640905-3640618 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References