Detailed information of TA system TA124225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 50055..50580
• Replicon: plasmid PNCS014853 strain 14[5]12:i:-
• Accession: NZ_CP039715
• Organism: Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain PNCS014853

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: E5N94_RS24580
• Protein ID: WP_001159863.1
• Coordinates: 50275..50580 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: E5N94_RS24575
• Protein ID: WP_000813641.1
• Coordinates: 50055..50273 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5N94_RS24545	45342..45723	+	382	Protein_50	NirD/YgiW/YdeI family stress tolerance protein	-
E5N94_RS24550	46000..46336	+	337	Protein_51	site-specific integrase	-
E5N94_RS24555	46595..47584	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
E5N94_RS24560	48078..48374	-	297	WP_001687482.1	hypothetical protein	-
E5N94_RS24570	48858..49379	-	522	WP_010999942.1	hypothetical protein	-
E5N94_RS24575	50055..50273	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E5N94_RS24580	50275..50580	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
E5N94_RS24585	50582..50872	+	291	WP_001266176.1	hypothetical protein	-
E5N94_RS24590	50869..51390	+	522	WP_000198608.1	hypothetical protein	-
E5N94_RS24595	51425..52207	+	783	WP_000082169.1	site-specific integrase	-
E5N94_RS24600	52216..52929	+	714	Protein_60	EAL domain-containing protein	-
E5N94_RS24610	52933..53442	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
E5N94_RS24615	53436..53921	+	486	WP_000905606.1	membrane protein	-
E5N94_RS24620	54198..54485	-	288	WP_071530243.1	hypothetical protein	-
E5N94_RS24625	54641..55201	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93838	93838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T124225 WP_001159863.1 NZ_CP039715:50275-50580 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T124225 NZ_CP039715:50275-50580 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT124225 WP_000813641.1 NZ_CP039715:50055-50273 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT124225 NZ_CP039715:50055-50273 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6