Detailed information of TA system TA124222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2603996..2604603
• Replicon: chromosome
• Accession: NZ_CP039710
• Organism: Thermoactinomyces vulgaris strain 2H

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A151YVK1
• Locus tag: FA954_RS13350
• Protein ID: WP_037998060.1
• Coordinates: 2603996..2604346 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FA954_RS13355
• Protein ID: WP_175413226.1
• Coordinates: 2604352..2604603 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA954_RS13325	2599643..2600923	+	1281	WP_037998052.1	Glu/Leu/Phe/Val dehydrogenase	-
FA954_RS13330	2601096..2601881	-	786	WP_062843382.1	RNA polymerase sigma factor SigB	-
FA954_RS13335	2601844..2602332	-	489	WP_037998054.1	anti-sigma B factor RsbW	-
FA954_RS13340	2602329..2602664	-	336	WP_037998057.1	STAS domain-containing protein	-
FA954_RS13345	2602661..2603722	-	1062	WP_052186901.1	PP2C family protein-serine/threonine phosphatase	-
FA954_RS13350	2603996..2604346	-	351	WP_037998060.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FA954_RS13355	2604352..2604603	-	252	WP_175413226.1	ribbon-helix-helix protein, CopG family	Antitoxin
FA954_RS13360	2604827..2606008	-	1182	WP_037998078.1	alanine racemase	-
FA954_RS13365	2606028..2606525	-	498	WP_131470884.1	hypothetical protein	-
FA954_RS13370	2606566..2607546	-	981	WP_138615196.1	outer membrane lipoprotein carrier protein LolA	-
FA954_RS13375	2607642..2609180	-	1539	WP_121874496.1	NAD(P)H-hydrate dehydratase	-
FA954_RS13380	2609223..2609603	-	381	WP_037997131.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12939.02 Da
• Isoelectric Point: 5.6976

>T124222 WP_037998060.1 NZ_CP039710:c2604346-2603996 [Thermoactinomyces vulgaris]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKAYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMNRVNEALMISLGMINF

Nucleotide

• Length: 351 bp

>T124222 NZ_CP039710:c2604346-2603996 [Thermoactinomyces vulgaris]
TTGATTGTGAAACGTGGCGATGTTTATTTTGCTGATCTATCCCCTGTGGTCGGATCCGAACAAGGTGGGGTTCGGCCTGT
TTTGGTGATTCAAAATGATATTGGCAATCGGTTCAGCCCCACCGTGATCGTCGCCGCGATTACAGCTCAAATTCAAAAGG
CCAAATTACCTACGCATGTGGAAATCGATGCCAAAGCATACGGGTTTGATCGTGACTCGGTCATATTGTTGGAACAAATC
CGAACGATCGATAAGCAAAGGCTGACGGATAAAATCACTCATCTGGATGATGAAATGATGAATCGTGTCAATGAAGCGTT
AATGATCAGTTTGGGTATGATTAACTTCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9737.22 Da
• Isoelectric Point: 4.8898

>AT124222 WP_175413226.1 NZ_CP039710:c2604603-2604352 [Thermoactinomyces vulgaris]
MISLPTKLLQEVDGVVARENSNRSEFIRQAMRMYLQERKKRLIREMMQRGYMEMARINLNIASEAFEAEEEAEDTLDRLV
SGV

Nucleotide

• Length: 252 bp

>AT124222 NZ_CP039710:c2604603-2604352 [Thermoactinomyces vulgaris]
ATGATCAGCCTGCCAACAAAATTGCTTCAAGAAGTGGATGGCGTTGTGGCGAGGGAAAATTCCAATCGGAGTGAGTTTAT
CCGCCAAGCGATGAGAATGTATCTCCAAGAGCGGAAAAAGCGTCTGATTCGTGAAATGATGCAACGCGGCTACATGGAGA
TGGCACGCATCAATCTCAACATCGCTTCGGAAGCATTTGAGGCAGAAGAAGAGGCTGAAGATACTTTGGATCGATTGGTT
AGTGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A151YVK1