Detailed information of TA system TA124221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 936517..937118
• Replicon: chromosome
• Accession: NZ_CP039710
• Organism: Thermoactinomyces vulgaris strain 2H

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FA954_RS04850
• Protein ID: WP_121874194.1
• Coordinates: 936517..936846 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A151YYH9
• Locus tag: FA954_RS04855
• Protein ID: WP_037994644.1
• Coordinates: 936846..937118 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA954_RS04845	934324..936036	-	1713	WP_138614901.1	hypothetical protein	-
FA954_RS13645	936123..936296	-	174	WP_156473333.1	hypothetical protein	-
FA954_RS04850	936517..936846	-	330	WP_121874194.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FA954_RS04855	936846..937118	-	273	WP_037994644.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FA954_RS04860	937320..938024	+	705	WP_138614902.1	YdcF family protein	-
FA954_RS04865	938016..938681	-	666	WP_138614903.1	hypothetical protein	-
FA954_RS04870	939108..940517	+	1410	WP_138614904.1	cytochrome ubiquinol oxidase subunit I	-
FA954_RS04875	940501..941517	+	1017	WP_037994635.1	cytochrome d ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-105)

Antitoxin

• SpoVT-AbrB domain (18-62)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12083.95 Da
• Isoelectric Point: 7.8862

>T124221 WP_121874194.1 NZ_CP039710:c936846-936517 [Thermoactinomyces vulgaris]
MAVPDRGDLVYVSFHPQAGHEQAGRRPAIVLSPQSFNAVTKFAVVCPITRKEKGYPFEVKLPDGLPIQGVILADQVKSLD
WQARQFTIKGRAPENVVNDCIELIHTFLS

Nucleotide

• Length: 330 bp

>T124221 NZ_CP039710:c936846-936517 [Thermoactinomyces vulgaris]
ATGGCCGTACCGGATCGCGGGGATCTTGTTTATGTCAGCTTCCATCCGCAAGCCGGGCATGAACAGGCCGGAAGACGCCC
CGCCATTGTCTTATCCCCCCAATCATTCAACGCAGTGACCAAATTCGCTGTGGTCTGCCCCATCACCCGCAAAGAAAAAG
GCTACCCATTTGAAGTGAAATTGCCCGACGGTCTGCCCATTCAAGGCGTCATTCTGGCGGACCAAGTCAAAAGCCTAGAT
TGGCAGGCACGGCAATTTACGATCAAAGGCCGGGCACCCGAAAACGTGGTTAATGATTGCATTGAATTAATCCATACCTT
TTTGTCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10264.92 Da
• Isoelectric Point: 6.4781

>AT124221 WP_037994644.1 NZ_CP039710:c937118-936846 [Thermoactinomyces vulgaris]
MKEEENQGGIMKMTTQVRKWGNSLAIRIPSHIAEMLSIKQGSELEMKVENQEIKLVPKKKKPTLDELLAKVTPENRHEEI
QLGTEGNELI

Nucleotide

• Length: 273 bp

>AT124221 NZ_CP039710:c937118-936846 [Thermoactinomyces vulgaris]
ATGAAAGAGGAGGAAAATCAAGGAGGAATCATGAAGATGACGACACAAGTCCGTAAATGGGGAAACAGCCTGGCCATTCG
GATCCCCAGCCATATTGCCGAGATGTTGTCCATCAAGCAAGGCTCCGAATTGGAAATGAAAGTGGAAAACCAAGAGATCA
AATTGGTTCCAAAGAAGAAAAAGCCCACATTGGATGAACTTCTGGCCAAAGTCACACCGGAAAACCGCCACGAGGAAATT
CAGCTTGGAACCGAAGGGAATGAGCTGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A151YYH9