Detailed information of TA system TA124217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1614182..1614699
• Replicon: chromosome
• Accession: NZ_CP039704
• Organism: Hankyongella ginsenosidimutans strain W1-2-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E6W36_RS09480
• Protein ID: WP_222872437.1
• Coordinates: 1614182..1614526 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E6W36_RS09485
• Protein ID: WP_246047473.1
• Coordinates: 1614523..1614699 (-)
• Length: 59 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6W36_RS09460 (E6W36_08950)	1609307..1610584	+	1278	WP_222872433.1	integrin alpha	-
E6W36_RS09465 (E6W36_08955)	1610482..1611906	+	1425	Protein_2015	cadherin domain-containing protein	-
E6W36_RS18710	1611916..1612395	+	480	WP_246048302.1	integrin alpha	-
E6W36_RS20640	1612283..1613461	+	1179	WP_222872435.1	hypothetical protein	-
E6W36_RS09475 (E6W36_08965)	1613600..1614136	-	537	WP_222872436.1	histidine phosphatase family protein	-
E6W36_RS09480 (E6W36_08970)	1614182..1614526	-	345	WP_222872437.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E6W36_RS09485 (E6W36_08975)	1614523..1614699	-	177	WP_246047473.1	antitoxin MazE family protein	Antitoxin
E6W36_RS09490 (E6W36_08980)	1614772..1615518	-	747	WP_222872439.1	adenosylcobinamide-GDP ribazoletransferase	-
E6W36_RS18725	1615515..1615586	-	72	WP_246048304.1	hypothetical protein	-
E6W36_RS09495 (E6W36_08985)	1615664..1616389	-	726	Protein_2023	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
E6W36_RS18730	1616386..1616499	-	114	WP_246047475.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
E6W36_RS18735	1616496..1616687	-	192	WP_246047477.1	hypothetical protein	-
E6W36_RS09500 (E6W36_08990)	1616672..1617928	-	1257	WP_246047479.1	cobyric acid synthase	-
E6W36_RS09505 (E6W36_08995)	1617925..1618530	-	606	WP_222872440.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-
E6W36_RS18740	1618545..1619309	-	765	WP_246048305.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (1-57)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12435.41 Da
• Isoelectric Point: 9.2944

>T124217 WP_222872437.1 NZ_CP039704:c1614526-1614182 [Hankyongella ginsenosidimutans]
MRRGEIRTVAGGKDYAGKPRPAVIVQDDLFDATDSVTLCAFTTDETDAPLFRLPVQPSPGNGLRAPCRLMVDKITTVHKT
KIGPAIGRLEDDDMLRLNRAMLVFLGLAASPRSR

Nucleotide

• Length: 345 bp

>T124217 NZ_CP039704:c1614526-1614182 [Hankyongella ginsenosidimutans]
ATGAGGCGCGGCGAAATCCGTACGGTTGCAGGCGGCAAGGACTATGCCGGCAAACCGCGCCCGGCGGTGATCGTGCAGGA
TGACCTGTTCGATGCGACCGACTCCGTCACGCTGTGCGCCTTCACCACCGATGAGACCGATGCGCCGCTGTTTCGGCTGC
CCGTGCAGCCCAGTCCCGGCAACGGCCTGCGCGCGCCCTGCCGCCTGATGGTCGACAAGATCACCACGGTTCACAAGACC
AAGATCGGCCCCGCGATCGGCCGACTGGAAGATGACGACATGCTGCGCCTCAACCGGGCGATGCTGGTGTTCCTGGGGCT
CGCCGCCAGCCCACGCAGCCGCTAG

Antitoxin

Protein

• Length: 59 a.a.
• Molecular weight: 6477.20 Da
• Isoelectric Point: 11.9559

>AT124217 WP_246047473.1 NZ_CP039704:c1614699-1614523 [Hankyongella ginsenosidimutans]
VRAHRARLRAQGLRPIQIWVPDVRAASFRAEAHRQSQAVASSRQAHDDQSFIDAVSDA

Nucleotide

• Length: 177 bp

>AT124217 NZ_CP039704:c1614699-1614523 [Hankyongella ginsenosidimutans]
GTGCGCGCGCATCGGGCCCGGCTGCGGGCGCAGGGGCTGCGACCGATCCAGATCTGGGTTCCGGACGTCCGGGCAGCATC
GTTTCGGGCCGAGGCGCATCGTCAGTCGCAGGCAGTGGCGAGCAGCCGGCAGGCGCATGACGACCAAAGCTTCATCGACG
CCGTGTCCGACGCATGA