Detailed information of TA system TA124214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 75324..75843
• Replicon: chromosome
• Accession: NZ_CP039701
• Organism: Vibrio cyclitrophicus strain ECSMB14105

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0L1L9L7
• Locus tag: FAZ90_RS15520
• Protein ID: WP_010429015.1
• Coordinates: 75562..75843 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K5UYY9
• Locus tag: FAZ90_RS15515
• Protein ID: WP_004730320.1
• Coordinates: 75324..75572 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FAZ90_RS15465	70398..70949	-	552	WP_046208926.1	hypothetical protein	-
FAZ90_RS15470	71084..72028	-	945	WP_016785137.1	RDD family protein	-
FAZ90_RS15480	72537..72989	-	453	WP_046208925.1	hypothetical protein	-
FAZ90_RS15495	73523..73876	+	354	WP_046208924.1	membrane protein	-
FAZ90_RS15500	74090..74392	-	303	WP_046208923.1	hypothetical protein	-
FAZ90_RS15505	74563..75129	-	567	WP_010429033.1	hypothetical protein	-
FAZ90_RS15510	75157..75249	-	93	WP_010429030.1	DUF3265 domain-containing protein	-
FAZ90_RS15515	75324..75572	+	249	WP_004730320.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FAZ90_RS15520	75562..75843	+	282	WP_010429015.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FAZ90_RS15525	75954..76961	-	1008	WP_010429012.1	hypothetical protein	-
FAZ90_RS15530	77018..77110	-	93	WP_010429009.1	DUF3265 domain-containing protein	-
FAZ90_RS15535	77125..77571	-	447	WP_010429007.1	DUF4262 domain-containing protein	-
FAZ90_RS15540	77600..77716	-	117	WP_099166415.1	DUF3265 domain-containing protein	-
FAZ90_RS15545	77720..78145	-	426	WP_046209711.1	hypothetical protein	-
FAZ90_RS15550	78174..78266	-	93	WP_136952239.1	DUF3265 domain-containing protein	-
FAZ90_RS15555	78373..79260	+	888	WP_046209703.1	site-specific integrase	-
FAZ90_RS15560	79257..80309	+	1053	WP_046209702.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	73523..86465	12942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10917.91 Da
• Isoelectric Point: 10.7726

>T124214 WP_010429015.1 NZ_CP039701:75562-75843 [Vibrio cyclitrophicus]
MTYKLDFKKSALKEWKKLGSTLQQQFKKKLIERLDNPHVPASKLSGADNMYKIKLRQSGYRLVYKVEDDVIIVTVLAVGK
RERSDVYRKAMKR

Nucleotide

• Length: 282 bp

>T124214 NZ_CP039701:75562-75843 [Vibrio cyclitrophicus]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCAACGCTTCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCGCATGTTCCGGCTTCAAAACTCTCTGGAGCTGACAACATGTATAAGATTA
AGCTGCGCCAATCGGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCAGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGTAAAGCTATGAAAAGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9109.39 Da
• Isoelectric Point: 3.9610

>AT124214 WP_004730320.1 NZ_CP039701:75324-75572 [Vibrio cyclitrophicus]
MTTRILADVAASITELKANPMKVATSAYGEPVAVLNRNEPAFYCVPAEAYEMMMDRLEDLELLTIAKERESEESISVNID
DL

Nucleotide

• Length: 249 bp

>AT124214 NZ_CP039701:75324-75572 [Vibrio cyclitrophicus]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACTGAGTTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTACGGTGAACCTGTTGCTGTACTGAACCGAAATGAGCCAGCATTTTATTGCGTACCAGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTTACTATTGCGAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A0L1L9L7

Antitoxin

• AlphaFold DB: A0A0L1LA33