Detailed information of TA system TA124210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 196550..197081
• Replicon: plasmid pABRDHK234
• Accession: NZ_CP039699
• Organism: Novosphingobium sp. ABRDHK2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: FA702_RS22555
• Protein ID: WP_004212690.1
• Coordinates: 196550..196831 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: FA702_RS22560
• Protein ID: WP_004212689.1
• Coordinates: 196818..197081 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA702_RS22530	192110..192838	-	729	WP_025549137.1	IS21-like element helper ATPase IstB	-
FA702_RS22535	192825..193370	-	546	Protein_193	IS21 family transposase	-
FA702_RS22540	193438..194307	-	870	WP_136958213.1	IS21-like element helper ATPase IstB	-
FA702_RS22545	194304..195809	-	1506	WP_136958214.1	IS21 family transposase	-
FA702_RS23050	196020..196478	+	459	WP_210417679.1	hypothetical protein	-
FA702_RS22555	196550..196831	-	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
FA702_RS22560	196818..197081	-	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FA702_RS22565	197465..197902	-	438	WP_007683347.1	metal-binding protein	-
FA702_RS22570	197982..199223	-	1242	WP_007683345.1	MFS transporter	-
FA702_RS22575	199217..200203	-	987	WP_009823975.1	copper resistance protein B	-
FA702_RS22580	200200..201906	-	1707	WP_136958154.1	copper resistance system multicopper oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..210225	210225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T124210 WP_004212690.1 NZ_CP039699:c196831-196550 [Novosphingobium sp. ABRDHK2]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T124210 NZ_CP039699:c196831-196550 [Novosphingobium sp. ABRDHK2]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT124210 WP_004212689.1 NZ_CP039699:c197081-196818 [Novosphingobium sp. ABRDHK2]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT124210 NZ_CP039699:c197081-196818 [Novosphingobium sp. ABRDHK2]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATAGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968